Cartilage Repair Models

Lunatum Biotech offers sophisticated, established, valid, and reproducible in-vitro and in-vivo models for cartilage regeneration:

in-vitro:

- 2D and 3D culture of mesenchymal stem cells, and cartilage cells, single culture or co-culture system, using cells from various species, including human cells; 

- tissue explant culture, in-vitro simulation of a joint (mini joint), or use and culture of regenerative tissue material for (trans-)differentiation studies using tissue from various species 

in-vivo:

Focal cartilage defect animal models (mouse, rat, rabbit, minipig, sheep):

 partial thickness cartilage defects: 

In the PTCD model, a partial thickness defect is created surgically in the upper cartilage layer in a joint.

full thickness cartilage defects:

The FTCD model involves the surgical creation of a focal defect with respect to all cartilage layers but sparing the subchondral bone.

osteochondral defects:

In the OCD model, a defect in all cartilage layers and the subchondral bone is created by a joint surgery procedure.

Evaluation:

Lunatum Biotech offers an array of the most widely accepted and meaningful evaluation methods for cartilage repair, applying PCR, NGS, Western blotting, ELISA, histology, immunohistochemistry, CT, MRI, IVIS, biomechanical testing, pain assessment, gait analysis and more ...