in-vitro And in-vivo VECTOR (LIBRARY) screening

Let us guide you in identifying vector candidates with optimal tropisms for musculoskeletal applications. Whether you're focusing on combating osteoarthritis, osteoporosis, rare bone diseases or other debilitating musculoskeletal disorders, our expertise is at your service.

With our proven protocols and extensive experience in preclinical gene therapy testing, we're equipped to pinpoint the ideal vector candidate for targeted payload delivery. From macroscopic musculoskeletal tissues to the intricate cellular level, we ensure precision in your therapeutic endeavors.

Partner with Lunatum Biotech and embark on the journey towards innovative solutions for musculoskeletal health.

Our Vector Screening Service Packages include:

Vector library screening: 

• consulting / development of project designs
• project management
• preparation of animal protocols 
• injection of the vector library or single vectors in mice, rats, rabbits, pigs and / or in non-human primates (AAALAC accredited facilities) 
• tissue harvest of on-target and off-target tissues
• isolation and purification of on-target and off-target cells
• DNA and RNA extraction from on-target and off-target cells and bulk tissues
• in-vivo biodistribution analysis of enriched barcoded vectors
• identification of lead candidates by Next Generation Sequencing and bioinformatic analysis
• several iterative rounds of in-vivo vector screening/selection
• detailed data analysis
• data reporting

Single vector testing:

• consulting / development of project designs
• project management
• in-vitro experiments: transduction/transfection efficiency using cartilage cells (chondrocytes), cells of the synovium (synoviocytes), bone cells (osteocytes). or tendon cells (tenocytes) from various species including human cells; determination of mouse/rat/pig/NHP to human ratio (interspecies variation)
• in-vitro experiment: assesssment of biocompatibility, inflammatory response, cell viability, toxicology using various cells types (as mentioned above)
• preparation of animal protocols 
• in-vivo studies: administration/injection of the vector library or single vectors in mice, rats, rabbits, pigs and / or in non-human primates (AAALAC accredited facilities) 
• in-vivo studies: biodistribution and safety evaluation in various animal models: e.g. intra-articular distribution, joint residence time, drug efflux rate from joint, joint histopathology, full body biodistribution and histopathology, pharmacokinetics
• In-vivo studies: efficacy evaluation in various animal models: cartilage/bone/tendon regeneration, synovial inflammation score, biomarker analysis, pain related outcome, joint function and gait analysis
• detailed data analysis
• data reporting